{"id":520,"date":"2016-09-26T07:34:14","date_gmt":"2016-09-26T04:04:14","guid":{"rendered":"http:\/\/ebnsinagenelab.com\/?page_id=520"},"modified":"2016-09-26T11:05:18","modified_gmt":"2016-09-26T07:35:18","slug":"%d8%b1%d8%b2%d9%88%d9%85%d9%87-%d8%af%da%a9%d8%aa%d8%b1-%d9%85%d8%ad%d9%85%d9%88%d8%af-%d8%b4%da%a9%d8%a7%d8%b1%db%8c-%d8%ae%d8%a7%d9%86%db%8c%d8%a7%d9%86%db%8c","status":"publish","type":"page","link":"https:\/\/ebnsinagenelab.com\/?page_id=520","title":{"rendered":"\u0631\u0632\u0648\u0645\u0647 \u062f\u06a9\u062a\u0631 \u0645\u062d\u0645\u0648\u062f \u0634\u06a9\u0627\u0631\u06cc \u062e\u0627\u0646\u06cc\u0627\u0646\u06cc"},"content":{"rendered":"
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\n

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\n
Curriculum Vitae<\/strong>
\n Dr Mahmoud Shekari Khaniani, M.D, Ph.D in Human Genetics<\/strong><\/div>\n
\n
\n

Personal details:<\/strong>
\nAddress of correspondence:
\n\u2022 Department of Medical Genetic
\nfaculty of Medicine
\nTabriz university of Medical Sciences
\nGolgasht Ave, Tabriz, I,R,Iran\u2022 Tel of organization : +98 413 3371587 —- +98 413 3375540
\n\u2022 Facsimaile Number: +98 413 3371587 —- +98 413 3375540
\nE-Mail : mahmoud.khaniani@gmail.com<\/span><\/a><\/span><\/p>\n<\/div>\n

\n
\n<\/div>\n
Education:<\/strong>
\n\u06f2\u06f0\u06f0\u06f4 – \u06f2\u06f0\u06f0\u06f7: Human Genetics<\/strong>, Doctorate (PhD)<\/strong>
\nPaediatric Department, Faculty of Medicine, Dentistry and Health Sciences, Cyto-Molecular Research Group, Murdoch Children research Institute, Royal children\u2019s Hospital, University of Melbourne, Australia<\/span>
\nThesis Title:<\/strong> Molecular characterization and assessment of clinical significance of small Fragile X alleles
\nSupervisor:<\/strong> Professor Andy Choo and A\/Pro Howard Slater1987-1994: Medical Doctor (M.D)<\/strong>
\nFaculty of Medicine, Tabriz University of Medical science, Tabriz, Iran<\/span><\/div>\n
\n
\n

Clinical and work Experience:<\/strong>
\n\u2022 Associate Professor in Human Genetics at the Department of Medical Genetic, Tabriz university of Medical Science (2008 so far )
\n\u2022 Director of Ebna cina Medical Genetic Diagnostic Centre, Specialized and Sub specialized Outpatient Clinic of Tabriz University of Medical Sciences, No 507, Sheikh Alraeis , Azadi Ave, Tabriz, Tran
\n\u2022 Clinical Genetic Consoler in Specialized and Sub specialized Outpatient Clinic of Tabriz University of Medical Sciences ( 2008 so far )
\n\u2022 Working in hospitals as a General Practitioner
\n\u2022 working in health centres as a Family Physician<\/p>\n<\/div>\n

\n
\n<\/div>\n
\n

Research Experience:<\/strong>
\n\u2022 Association study of SLC6A4 gene and Autism patients in north west of Iran (2016- in progress)
\n\u2022 Association study of mutated FMR1 alleles and Autism patients in north west of Iran.(2010-2013)
\n\u2022 Detaection of Haemaophillia A carriers in Azarei Turkish Population of Iran. (2010-2013)
\n\u2022 Expression of cDNA human F8 gene from Recombinant DNA clone in hamster ovary cells (2010 – in progress )
\n\u2022 Production of Human Epidermal Growth Factor (EGF) from Recombinat DNA clone in Escherichia coli and Yeast (2010 – in progress )
\n\u2022 Production of Vascular Epidermal Growth Factor (VEGF) from Recombinat DNA clone in Escherichia coli and Yeast (2012 – in progress )
\n\u2022 Production of Human Stem Cell Factor (SCF) from Recombinat DNA clone in Escherichia coli and Yeast (2010- in progress )
\n\u2022 Extraction of Human CD34 + cells from Bone Marrow (2011- in progress )
\n\u2022 Molecular characterization and assessment of clinical significance of small Fragile X alleles (2004-2006)
\n\u2022 Investigation of tuberculosis of urinary duct system<\/p>\n

\n

Experimental Techniques:<\/strong>
\nMolecular and cell biology<\/span>
\n\u2022 Routine bacterial cell culture technique (bacterial cultivation, preparation and transformation of competent cells)
\n\u2022 Routine DNA manipulation techniques (cloning, plasmid and bacterial artificial chromosome construction, transfection).
\n\u2022 DNA, RNA extraction from bacterial and mammalian cells and tissues
\n\u2022 Polymerase chain reaction, PCR, (amplification including long range and screening), sequencing, revers transcription and real-time PCR
\n\u2022 Manipulation of large BACs by purification, digestion and transfection)
\n\u2022 PFGE and agars gel electrophoresis (DNA)
\nTissue Culture<\/span>
\n\u2022 Culture of lymphocytes
\n\u2022 Culture of Amniotic fluid
\n\u2022 Routine cell culture and passage
\n\u2022 Freezing stocks of mammalian cells
\n\u2022 Thawing of frozen mammalian cells
\n\u2022 Primary culturing mammalian cells (fibroblast, kidney, brain, liver\u2026)
\n\u2022 Maintenances of cell line cultures
\n\u2022 Counting cells and calculation of cell size using hemocytometer
\n\u2022 DNA transfection into human cells and produce stable cell line
\n\u2022 Microscopy (phase contrast, fluorescent and confocal)
\n\u2022 Media preparation
\n\u2022 Experience with tissue collection and biopsies (human and mouse)
\n\u2022 Flow cytometry
\n\u2022 Isolation of liver stem cells from mouse embryo
\n\u2022 Isolation of heamopotic stem cells from adult mouse
\nCommunication<\/strong><\/span>
\n\u06f1\u066b Knowledge of English language, Reading, Writing, speaking and listening
\n\u06f2\u066b Preparation of scientific poster presentation and paper
\n\u06f3\u066b Presentations at laboratory meetings and journal clubs
\n\u06f4\u066b Familiarity with computer programs such as word, PowerPoint, Excel, experienced in database searches<\/p>\n

\n

Teaching experiences<\/strong>
\n\u2022 Medical Genetics for medical students
\n\u2022 Human Cryogenetics for Msc student
\n\u2022 Medical Genetics for Msc and Ph.D student
\n\u2022 Molecular Genetics for Msc and Ph.D student
\n\u2022 Genetic engineering for Msc and Ph.D student
\n\u2022 Advanced Molecular Genetics for Msc and Ph.D student<\/p>\n

\n

Presentation:<\/strong>
\n\u06f5\u066b Poster presentation in 4th Australasian Gene therapy Society Meeting, Melbourne, Australia (April, 2005
\n\u06f6\u066b Poster presentation in The 11th International Congress of Human Genetics, Brisbane, Australia (6th -10th August, 2006)
\n\u06f7\u066b Poster and oral presentation in 13th Australian Society of Cytogeneticists (ASoC) and 1th Molecular Genetics Society of Australasia (MGSA)Interim Scientific Meeting, (16th -18th March 2007, Sydney )
\n\u06f8\u066b Poster presentation in 11th International Conference in Human genetics,( 16th -19th June, Nice, France)
\n\u06f9\u066b Poster presentation in 13th International Workshop on Fragile X and X-linked Mental Retardation (3th -6th October 2007, Venezia Lido, Ital<\/span>y) : Pathology of the intermediate size (grey zone) FMR1 gene\u2019s alleles-facts or fiction? Danuta Z Loesch, Mahmoud S khaniani, Trent Burgess, Richard Huggins, Howard R Slator
\n\u06f1\u06f0\u066b Oral presentation in\u201d infertility and laparoscopy congress\u201d Tabriz university of medical science, September 2008
\n\u06f1\u06f1\u066b Poster presentation in \u201c \u06f1\u06f1th Iranian medical Genetics Congress \u201c May 2010 Tehran \u2013Iran<\/span> (An Improved diagnostic PCR assay for detection of \u03b1-thalassaemia deletions and \u03b1-globin gene triplication by multiplex polymerase chain reactions )\u201c
\n\u06f1\u06f2\u066b Poster presentation in \u201c \u06f1\u06f1th Iranian medical Genetics Congress \u201c May 2010- Tehran \u2013Iran<\/span> (Molecular Analysis of MEFV Gene in Iranian Patients with Familial Mediterranean Fever)
\n\u06f1\u06f3\u066b Poster presentation for the 3rd International and 18th National Congress of Iranian Society for Reproductive Medicine Tabriz, Iran 18-20 April 2012<\/span> \u201cAn infertile man with a 45X karyotype (case report)\u201d
\n\u06f1\u06f4\u066b Poster presentation in European Human Genetics Conference N\u00fcrnberg, Germany June 23-26, 2012<\/span> \u201cControversy in the mode of inheritance in Familial Mediterranean fever diseases; Molecular Analysis of MEFV Gene in Patients\u201d
\n\u06f1\u06f5\u066b Poster presentation in European Human Genetics Conference N\u00fcrnberg, Germany June 23-26, 2012 \u201cSodium butyrate and Valproic acid as a splicing restoring agents in erythroid cells of \u03b2-thalassaemic patients\u201d<\/p>\n

\n

Publication:<\/strong><\/p>\n

\u06f1\u066b Mahmoud, S Khaniani<\/strong> , Paul Kalitsis, Trent Burgess, Howard R Slater An Improved Diagnostic PCR Assay for Identification of Cryptic Heterozygosity for CGG Triplet Repeat Alleles in the Fragile X Gene (FMR1). Journal of Molecular Cytogenetics<\/strong><\/em> 2008; 1:5 Page 1-6<\/span>
\n\u06f2\u066b Ruimei Hu, Nicole E. Buck, Mahmoud S. Khaniani<\/strong>, Leonie Wood, Hady Wardan, Jean-Francois Benoist, Lingli Li, Jim Vadolas, Joseph P. Sarsero, Panos A. Ioannou, Heidi L. Peters (Three first investigators contributed equally and should be considered as first authors); \u201cGene induction for the treatment of Methylmalonic acidouria \u201c The journal of gene Medicine<\/strong> , 2009, Volume 11, Issue 4, Page 361-369<\/span>
\n\u06f3\u066b Mahmoud, S Khaniani<\/strong> , P. Kalitsis, D.Z. Loesch, R J. Michell, A. Choo, H. R Slater Determination of Heterozygosity at the FMR1 gene by An Enhanced Polymerase Reaction Assay; European Journal of Human Genetics<\/em><\/strong>, 2007, Volume 15 supplement 1 ,June page109
\n\u06f4\u066b DZ Loescha, MS Khaniani<\/strong> , HR Slaterd,e, JP Rubiof, QM Buig, K Kotscheth and at all Small CGG repeat expansion alleles of FMR1 gene are associated with Parkinsonism Clinical Genetics<\/strong> 2009: 76: 471\u2013\u06f4\u06f7\u06f6<\/span>
\n\u06f5\u066b Danuta Z. Loesch, David E. Godler, Mahmoud Khaniani<\/span><\/strong>, Emma Gould and at all \u201cLinking the FMR1 Alleles With Small CGG Expansions With Neurodevelopmental Disorders: Preliminary Data Suggest an Involvement of Epigenetic Mechanisms\u201d American Journal of Medical Genetics ( Part A)<\/span><\/strong>, 2009;10:2306-2310
\n\u06f6\u066b A.G. Behbahan, B. Poorshiri, F. Mortazavi, M.S. Khaniani<\/strong> and S.M. Derakhshan
\n\u201cNPHS1 Gene Mutations in Children with Nephrotic Syndrome in Northwest Iran\u201d Pakistan Journal of Biological Sciences<\/strong> 16 (17): 882-886, 2013
\n\u06f7\u066b Ebrahimi Ammar, khaniani S Mahmoud<\/strong><\/span>, Mansoori Sima \u201dStable expression of modified Gene encoding functional Human coagulation Factor VIII\u201d
\nJournal for Drugs and medicines<\/strong>, 2011,Vol 3 (2), pp. 19-25
\n\u06f8\u066b Negar Saliani, Masoud Darabi, Bahman Yousefi, Behzad Baradaran, Mahmoud Shekari Khaniani<\/strong>, Maryam Darabi, Maghsod Shaaker, Amir Mehdizadeh, Tahereh Naji and Mehrdad Hashemi. \u201cPPAR\u03b3 agonist-induced alterations in \u0394\u06f6-desaturase and stearoyl-CoA desaturase 1: Role of MEK\/ERK1\/2 pathway\u201d
\nWorld Journal of Hepatology<\/strong>, 2013, 5(4): 220-225.
\n\u06f9\u066b Bahman Yousefi, Masoud Darabi, Behzad Baradaran, Mahmoud Shekari Khaniani<\/strong>, Mohammad Rahbani, Maryam Darabi, Shabnam Fayezi, Amir Mehdizadeh, Negar Saliani, Maghsod Shaaker \u201cInhibition of MEK\/ERK1\/2 Signaling Affects the Fatty Acid Composition of HepG2 Human Hepatic Cell Line\u201d Bioimpacts, 2012, 2(3), 145-150<\/strong>
\n\u06f1\u06f0\u066b \u0640Jamal Mohmmadian. Sima Mansoori Derakhshan , Masood Mohmmadian, Mahmoud Shekari Khaniani<\/strong>* \u201cConstruction of Yeast Recombinant Expression Vector Containing Human Epidermal Growth Factor (hEGF)\u201d
\nAdvanced Pharmaceutical Bulletin: 2013; 3(2), 473-476<\/strong>
\n\u06f1\u06f1\u066b Behrooz Farhadi, Mahmoud Shekari Khaniani<\/strong>, Sima Mansoori Derakhshan \u201cConstruction of pPIC9 Recombinant Vector Containing Human Stem Cell Factor\u201d Advanced Pharmaceutical Bulletin: 2013;3(2), 303-308<\/strong>
\n\u06f1\u06f2\u066b : Azizeh Farshbaf khalili, Mahnaz Shahnazi, Khadijeh bHajizadeh, Mahmoud Shekari Khaniani<\/strong> \u201cDown Syndrome Screening Methods In Iraniani Pregnant Women\u201d Journal of caing Scinces ,2012,(13),145-151<\/strong>
\n\u06f1\u06f3\u066b Salman Asghari, Mahmoud Shekari Khaniani<\/strong> , Masood Darabei, Sima Mansoori Derakhshan \u201cCloning of Soluble Human Stem Cell Factor in pET-26b(+) vector\u201d
\nAdvanced Pharmaceutical Bulletin, 2014, 4(1), 91-95<\/strong><\/p>\n

\u06f1\u06f4\u066b Sima Mansoori Derakhshan, Shamsei Abbasalizadeh, Fatemeh Abbasalizadeh, Mahmoud Shekari Khaniani *<\/strong> \u201cPernatal Diagnosis of Spinal Muscular Atrophy: Clinical experience and Moleculargenetics of SMN gene analysis in 36 cases\u201d
\nJournal of Prenatal Medicine, 2013 Vol: 7 (3); 32-34<\/strong>
\n\u06f1\u06f5\u066b Mahmoud Shekari Khaniani<\/strong>, Sima Mansoori Derakhshan \u201cMolecular Characterisation and Assessment of Clinical Significance of Small Fragile X Alleles\u201d Journal of Analytical Research in Clinical Medicine (Vol. 1, No. 1 Page 1-17)<\/strong>
\n\u06f1\u06f6\u066b Afkhamei F, Shekari Khaniani .M<\/strong>, Farzadei, Paknejade, Mansoori Derakhshan. S “The HLA- G 14bp insertion\/delation polymorphisim in women with recurrent spontaneous abortion\u201d Iranian Journal of Allergy, Asthma and Immunology. 2014; 13 (5): 364-369<\/strong>
\n\u06f1\u06f7\u066b Tamouchin Moharrami, Sima Mansoori Derakhshan, Abbas Ali H. Pourfeizi. Mahmoud Shekari Khaniani<\/strong> ” Detaection of Haemaophillia A carriers in Azarei Turkish Population of Iran Clinical and Applied Thrombosis \/hemostasis<\/strong> 2014
\n\u06f1\u06f8\u066b Nahid Karimian fathi\u0648 Mahmoud Shekari khaniani<\/strong>, Vahid Motazeri , Sima Mansoori Derakhshan “Minor role of BRCA2 mutation (Exone 2 and Exone 11) in patients with early- onset breast cancer amongst Iranian Azari-Turkish women “ Iranian Journal of Basic Medical Sciences, 2014 , Vol. 17 ,No 2<\/strong>
\n\u06f1\u06f9\u066b Samira Goldar, Mahmoud Shekari Khaniani<\/strong>, Sima Mansoori Derakhshan, Behzad Baradran ” Molecular mechanisms Apoptosis and Role in cancer development and treatment” Asian Pacific Journal of Cancer Prevention: APJCP 2015, 16 (6): 2129-44
\nIndex: ISI, PubMed Central, PubMed, Embase, Scopus, \u2026\u2026\u2026. IF: Impact factor: 2.514<\/strong><\/p>\n

\u06f2\u06f0\u066b Mahmoud Shekari Khaniani<\/strong>, Nasrin Sohrapi, Neda Mansoori Derakhshan. Sima Mansoori Derakhshan ” One novel frameshift mutation on exon 64 of COL7A1 gene in an Iranian individual suffering recessive Dystrophic epidermolysis Bullosa” Annals of Clinical and Laboratory Sciences Vol , 45 , no, 5 , 2015<\/strong>
\nIndex: ISI -pubmed, Scopus\u2026\u2026.. Impact factor :1<\/strong><\/p>\n

\u06f2\u06f1\u066b Nasrin Sohrabi, Mahmoud Shekari Khaniani<\/strong>, Sima Mansoori Derakhshan<\/span>” Evaluation of Association Between HLA Class II DR4\u2013DQ8 Haplotype and Type I Diabetes Mellitus in Children of East Azerbaijan State of Iran” Adv Pharm Bull, 2015, Volume 17, Issue 2, February 2015, Page 108-111
\nIndex: ISI, PubMed Central, PubMed, Embase, Scopus, IF: 0.875
\n\u06f2\u06f2\u066b Mahmoud Shekari Khaniani<\/span><\/strong>, Mahdieh Taghizadeh, Abbasali Hosseinpour Feizi, Sima Mansoori Derakhshan” Sodium Butyrate and Valproic Acid as Splicing Restoring Agents in Erythroid Cells of b-Thalassemic Patients” Iranian Journal of Biotechnology , Volume 14, Issue 1, Winter 2016, Page 9-15
\nIndex: ISI, PubMed Central, PubMed, Embase, Scopus, IF: 0.8
\n\u06f2\u06f3\u066b Sima Mansoori Derakhshan<\/strong>1 , Aziz Khorrami2, Abbasali Hosseinpour Pour Feizi and Mahmoud Shekari Khaniani “Spectrum of \u03b2-Globin Gene Mutations and \u03b2-Thalassemia Haplotype Analysis among the Iranian Azeri Turkish Population “ Epidemiology , 2015, Vol 5. Isuue 4<\/strong>
\nIndex: ISI, PubMed Central, PubMed, Embase, Scopus, IF: 0.875
\n\u06f2\u06f4\u066b Sima Mansoori Derakhshan , Fatemeh Zeinali Sehrig, Nasrin Sohrabi, Siamak Shiva,4 Behzad Baradaran, and Mahmoud Shekari Khaniani<\/span><\/strong> ” The Association between Human Leukocyte Antigen Class II DR3\u2013DQ2 Haplotype and Type 1 Diabetes in Children of the East Azerbaijan State of Iran” Iran Red Crescent Med J. 2015 Sep; 17(9)
\nIndex: ISI, PubMed Central, PubMed, Embase, Scopus, IF: 0.875
\n\u06f2\u06f5\u066b Mahmoud Shekari Khaniani<\/strong>, Sima Mansoori Derakhshan \u201cCytogenetic finding in patients with intellectual disability and\/or multiple congenital anomalies\u201d Journal of Analytical Research in Clinical Medicine (2016 ,Vol. 4, No. 2 Page 97-103)<\/strong>
\n\u06f2\u06f6\u066b Mahmoud Shekari Khaniani<\/strong> , Abdollah Ebrahimi, , Setareh Daraei, , Sima Mansoori Derakhshan ” Genotyping of Intron Inversions and Point Mutations in Exon 14 of the FVIII Gene in Iranian Azeri Turkish Families with Hemophilia A” Indian Journal of Hematology and Blood Transfusion, 2016<\/p>\n

\n

Membership:<\/strong>
\n\u2022 Australasian Gene Therapy Society
\n\u2022 Human Genetics Society of Australia
\n\u2022 Human Genetics Society of Iran<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

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